ALDEFLUOR™--用于检测和分离具有活性的干/祖细胞

ALDEFLUOR™ 简单、快速，用以检测具有活性的正常 或肿瘤干/祖细胞：

ALDEFLUOR™荧光试剂系统提供了一种不同于一般依靠细胞表型、 基于细胞内乙醛脱氢酶（ ALDH）活性对干/祖细胞进行鉴定、评估 和分离的方法。含有高表达 ALDH的细胞具有干/祖细胞的特性，这些细胞包括CD34+细胞、CD133+细胞、Kit+细胞、谱系抗原阴性 (Lin-)细胞、集落形成细胞、长期培养起始细胞以及NOD/SCID亚群细 胞。ALDEFLUOR™专为人血样品优化，目前也被应用于非造血谱系 以及多个物种的干/祖细胞鉴定。

为什么选择ALDEFLUOR™?

• 检测源自多种谱系细胞的正常/恶性前体细胞，包括造血、乳腺、头颈部、肺、肝、胰腺、子宫颈、卵巢、膀胱、前列腺、结肠和甲状腺等
• 无毒性，ALDHbr细胞可应用于下游分析
• 兼容免疫表型分析和其他荧光抗体对细胞的复染色
• 重复性高，操作简单，保质期长

ALDEFLUOR™的典型FACS结果图：

ALDEFLUOR™的工作原理：

• 不带电荷的ALDH-底物，BAAA（BODIPY®-氨基乙醛）通过被动 扩散进入活细胞。
• BAAA被细胞内的ALDH转化为带负电荷的反应产物BAA（BODIPY®-甘氨酸），该产物滞留在细胞内，使细胞高表达 ALDH，呈现明亮的荧光。
• 高表达ALDH（ALDHbr）的细胞带有明亮的荧光，在流式细胞仪的 绿色荧光通道（520-540 nm）被检出。
• 由于只有具有完整细胞膜的细胞才可以保留ALDEFLUOR™的反应 产物，因此 ALDEFLUOR™只鉴定具有活性的ALDHbr细胞。

操作流程：

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肿瘤干细胞：综述

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造血肿瘤细胞

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